Solvent structural study on deuterated sperm whale myoglobin by both X-ray and neutron diffractions
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چکیده
منابع مشابه
Electrostatic Interactions in Sperm Whale Myoglobin
The electrostatic free energy contribution to the stability of sperm whale ferrimyoglobin was evaluated according to the static accessibility modified TanfordKirkwood model. The electrostatic free energy contribution of each distinct structural element was divided into one term arising from interactions between it and other elements (interelemental) and another from interactions within the part...
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Reconstruction of the sperm whale myoglobin structure was accomplished by a series of aqueous condensations of suitably protected synthetic myoglobin fragments to a large fragment prepared from the native protein. Reaction of N alpha,N epsilon 19-acetimidomyoglobin with 3-bromo-2-(2-nitrophenylsulfenyl)skatole (BNPS-skatole) yielded the fragment corresponding to residues 15-153. The covalent st...
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The urea and guanidine hydrochloride (GuHCI) unfolding of sperm whale ferrimyoglobin and of horse ferrimyoglobin has been studied under equilibrium conditions at both neutral and acid pH. Using two spectral parameters (the Soret absorbance and the molar ellipticity at 222 nm) to monitor conformational equilibria, and various thermodynamic treatments of the resulting data, it was found that the ...
متن کاملEnhanced visibility of hydrogen atoms by neutron crystallography on fully deuterated myoglobin.
Although hydrogens comprise half of the atoms in a protein molecule and are of great importance chemically and structurally, direct visualization of them by using crystallography is difficult. Neutron crystallography is capable of directly revealing the position of hydrogens, but its use on unlabeled samples faces certain technical difficulties: the large incoherent scattering of hydrogen resul...
متن کاملProbing substates in sperm whale myoglobin using high-pressure crystallography.
Pressures in the 100 MPa range are known to have an enormous number of effects on the action of proteins, but straightforward means for determining the structural basis of these effects have been lacking. Here, crystallography has been used to probe effects of pressure on sperm whale myoglobin structure. A comparison of pressure effects with those seen at low pH suggests that structural changes...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 1993
ISSN: 0108-7673
DOI: 10.1107/s010876737809649x